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SAN FRANCISCO, Sept. 20 (Xinhua) -- Researchers in the United States have identified a key region within a protein that governs how accurately the gene-editing tool known as CRISPR-Cas9 homes in on a target deoxyribonucleic acid (DNA) sequence.
CRISPR is short for Clustered Regularly Interspaced Short Palindromic Repeats and Cas9 stands for CRISPR Associated Protein.
The researchers at the University of California Nike Air Max TN Plus Ultra Women's Black Pink , Berkeley, and Massachusetts General Hospital said they have tweaked the region within the Cas9 protein to produce a hyper-accurate gene editor with the lowest level of off-target cutting to date. Identified as a master controller of DNA cutting, the protein domain known as REC3 is a target for re-engineering to further improve accuracy and to minimize the chance that CRISPR-Cas9 will edit DNA at the wrong place.
Co-first author Janice Chen Nike Air Max Plus TN Ultra Women's White Pink , a graduate student in the lab of Jennifer Doudna, the UC Berkeley professor of molecular and cell biology who was one of the first scientists to invent CRISPR-Cas9 technology, noted in a press release that "we have found that even minor alterations in the REC3 domain of Cas9 affect the differential between on- and off-target editing Nike Air Max 97 Plus Men's Racer Pink Black , which suggests that this domain is an obvious candidate for in-depth mutagenesis to improve targeting specificity."
Targeting specificity is a key consideration when doing gene therapy in humans.
Currently, scientists using CRISPR-Cas9 create a single-guide ribonucleic acid, or sgRNA Nike Air Max Plus 97 Men's Blue White , an RNA molecule that includes a chain of 20 ribonucleic acids that complements a specific 20-nucleic-acid DNA sequence they want to target, and attach it to Cas9. This guide RNA allows Cas9 to home in on the complementary DNA, bind to it and cut the double stranded helix.
However Nike Air VaporMax Plus Men's Blue , the Cas9-sgRNA complex can bind to DNA that doesn't exactly match, leading to undesirable off-target cutting.
Doudna's lab discovered in 2015 a conformational switch of Cas9 that is activated when the RNA guide and DNA target match. They found that only when the RNA and DNA match closely does the 3D structure of Cas9, in particular the conformation of the HNH nuclease domain Nike Air VaporMax Plus Grape White Purple Men's , change and activate the scissors of C. Wholesale Authentic Jerseys Wholesale Cheap Hockey Jerseys China Wholesale Football Jerseys From China Wholesale College Basketball Jerseys Cheap Jerseys China Cheap Jerseys China Cheap Soccer Jerseys Cheap Hockey Jerseys Cheap Nike NFL Jerseys Cheap NCAA College Jerseys