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MessagePosté le: Mar 7 Aoû 2018 - 08:10    Sujet du message: The researchers at the University Répondre en citant

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SAN FRANCISCO, Sept. 20 (Xinhua) -- Researchers in the United States have identified a key region within a protein that governs how accurately the gene-editing tool known as CRISPR-Cas9 homes in on a target deoxyribonucleic acid (DNA) sequence.

CRISPR is short for Clustered Regularly Interspaced Short Palindromic Repeats and Cas9 stands for CRISPR Associated Protein.

The researchers at the University of California Nike Air Max TN Plus Ultra Women's Black Pink , Berkeley, and Massachusetts General Hospital said they have tweaked the region within the Cas9 protein to produce a hyper-accurate gene editor with the lowest level of off-target cutting to date. Identified as a master controller of DNA cutting, the protein domain known as REC3 is a target for re-engineering to further improve accuracy and to minimize the chance that CRISPR-Cas9 will edit DNA at the wrong place.

Co-first author Janice Chen Nike Air Max Plus TN Ultra Women's White Pink , a graduate student in the lab of Jennifer Doudna, the UC Berkeley professor of molecular and cell biology who was one of the first scientists to invent CRISPR-Cas9 technology, noted in a press release that "we have found that even minor alterations in the REC3 domain of Cas9 affect the differential between on- and off-target editing Nike Air Max 97 Plus Men's Racer Pink Black , which suggests that this domain is an obvious candidate for in-depth mutagenesis to improve targeting specificity."

Targeting specificity is a key consideration when doing gene therapy in humans.

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However Nike Air VaporMax Plus Men's Blue , the Cas9-sgRNA complex can bind to DNA that doesn't exactly match, leading to undesirable off-target cutting.

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